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Indirect immunofluorescence assays and confocal microscopy confirms μ1, μ2, RdRP, and eGFP depletion in induced trophozoites. BiP, tubulin, and CWP2 staining in non-induced and induced cells is identical. For μ1, RdRP, and eGFP, double transfections using the expression vector pTubApaHApac and the inducible dsRNAneo were performed. Images were processed in the same way. Bars = 10 μm.
