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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Parasitol. 2010 Aug;96(4):815–819. doi: 10.1645/GE-2406.1

FIGURE 4.

FIGURE 4

Long foreign dsRNA of μ1, μ2, and RdRP do not produce siRNA. Low-molecular-weight RNAs from the induced trophozoites were electrophoresed in SDS-Urea PAGE, blotted and probed using partially digested in vitro transcribed RNA (Hutvagner et al., 2000). In vitro transcribed 25nt-exoRNA for μ1 was mixed with trophozoite homogenate followed by low-molecular-weight RNA extraction and used as control (Ct). The polyacrylamide gel shows the quality and quantity of RNA (rRNA) by ethidium bromide staining (lower panel). RNA size markers in nucleotides are on the left.