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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Parasitol. 2010 Aug;96(4):815–819. doi: 10.1645/GE-2406.1

FIGURE 5.

FIGURE 5

Indirect immunofluorescence assays and confocal microscopy show that ESCP-HA and CLH are retained or excluded from the place of sorting, respectively, when μ1 expression is reduced (ESCPI and CLHI). No alteration in the subcellular localization of μ2 or VSP9B10 is observed in induced cells (μ2I and VSP9B10I). The mAbs anti-HA, 2F5 (Rivero et al., 2010), and anti-9B10 (Nash et al., 2001), were employed as primary Ab to detect ESCP, μ2, and VSP9B10, respectively. GiCLH polyclonal antibody was used for detection of clathrin (Marti et al., 2003).