. Author manuscript; available in PMC: 2011 Aug 1.

Published in final edited form as: J Parasitol. 2010 Aug;96(4):815–819. doi: 10.1645/GE-2406.1

Table I. Effect of siRNA and dsRNA on targeted gene expression.

Gene Target	dsRNA	mRNA (% of reduction)	Immunoblotting (% protein reduction)	IFA (% protein reduction)	% reduction on cell growth	% reduction cyst	Effect over Related Proteins
BiP	_exosRNA	0.1¹
	_endsRNA	0.2¹
	_enddsRNA	0.8¹^,²	-	NE	ND	ND	ND
Alpha tubulin	_exosRNA	0.1¹
	_endsRNA	0.1¹
	_enddsRNA	0.4¹^,²	15	NE	NE	ND	ND
μ1	_exosRNA	0.1¹
	_endsRNA	0.5¹
	_enddsRNA	66.5¹^,²	70	80	NE	83 (Touz et al., 2004)	ESCP, AcPh, CLH^a
μ2	_exosRNA	0.1¹
	_endsRNA	0.5¹
	_enddsRNA	99.2¹^,²	99	90	68 (Rivero et al., unpublished)	98 (Rivero et al., unpublished)	LDL^b
RdRP	_exosRNA	0.1¹
	_endsRNA	0.1¹
	_enddsRNA	37.2¹^,²	ND	60	NE	ND	NE^c
eGFP	_exosRNA	0.1¹
	_endsRNA	0.2¹
	_enddsRNA	47.2¹	90	80	NE	ND	NE^d
CWP2	_exosRNA	0.1¹
	_endsRNA	0.3¹
	_enddsRNA	22.8¹^,²	40	NE	NE	NE	NE^e

Determined by semiquantitative RT-PCR.

Determined by Slot-blot and densitometry analysis using Gel-Pro Analyzer version 4.5.

NE: no effect detected.

ND: not determined.

The encystation-specific cysteine protease ESCP, the acid phosphatase AcPh, and the clathrin heavy chain CLH were analyzed by IFA in μ1-depleted trophozoites, and showed alterations in their subcellular localizations.

Bodipy-LDL was not internalized in μ2-depleted trophozoites (Rivero et al, submitted).

No effect on VSP expression was found. No other proteins were analyzed.

No effect was found on ESCP, BiP, μ2, CWP1, or CWP2 expression or localization.

No effect was found on CWP2, CWP1, or BiP expression or localization.