Figure 2.
Pretreatment with GDNF inhibited microglia activation in the slice culture. A. Representative pictures of OX-6-positive microglia in the substantia nigra area upon LPS stimuli and pretreatment with GDNF (DIV16). LPS at 10, 20, 40, and 80µg/ml (A–c, d, e, f) increased the number of OX-6 positive microglia and pretreatment with GDNF (A–g, h, j) inhibited microglia activation. B. Graph representing LPS-induced microglia activation and inhibition by GDNF. LPS induced microglia activation in a dose-dependent manner and GDNF pretreatment inhibited microglia activation except at LPS at 40µg/ml (**p<0.01 or ***p<0.001 control versus LPS; #p<0.05 or ##p<0.01 LPS versus GDNF plus LPS, where n = 6/group).
