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. Author manuscript; available in PMC: 2011 Aug 25.
Published in final edited form as: J Neuroimmunol. 2010 May 14;225(1-2):43–51. doi: 10.1016/j.jneuroim.2010.04.010

Figure 4.

Figure 4

GDNF reduced LPS-induced suppression of PI3K expression. The slice cultures were treated with GDNF and/or LPS (20µg/ml) at DIV13, and protein samples were immunoblotted with polyclonal PI3K antibody after 1hr and 72hr treatment, respectively. A. Representative bands of PI3K expression at 1hr and 72hr time-points. There was no significant difference between control and the other groups at 1hr. However, at 72hr the suppression of PI3K expression by LPS was observed, and pretreatment with GDNF reversed its inhibition. B. Graph showing optical density of PI3K bands demonstrated GDNF prevented LPS-induced decrease in PI3K expression. (*p<0.05 control versus LPS; #p<0.05 LPS versus GDNF plus LPS, where n = 3).