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. Author manuscript; available in PMC: 2011 Aug 25.
Published in final edited form as: J Neuroimmunol. 2010 May 14;225(1-2):43–51. doi: 10.1016/j.jneuroim.2010.04.010

Figure 5.

Figure 5

GDNF abolished the inhibition of PI3K by LPS in both primary neuronal culture and microglia. Primary microglia culture was treated with LPS (1µg/ml) for 72hr, the conditioned medium was fed into primary neuronal-enriched culture and primary microglia culture for 10min and 1hr, followed by the immunoblotting of PI3K. A. Immunoblotting bands of PI3K from microglia and neuronal culture. No significant difference was found after 10min treatment. LPS-treated conditioned medium suppressed the expression of PI3K after 1hr treatment in both different cultures, and pretreatment with GDNF (10ng/ml) 1hr before markedly inhibited its inhibition. B. Optical density of PI3K bands showed GDNF prevented the LPS-induced decrease in PI3K expression in microglia. C. GDNF prevents the LPS-induced inhibition of PI3K expression in neurons. (**p<0.01 control versus LPS; #p<0.05 LPS versus GDNF plus LPS, n = 3).