Skip to main content
. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Exp Cell Res. 2010 Jul 13;316(16):2644–2653. doi: 10.1016/j.yexcr.2010.06.028

Figure 7. FN knockdown inhibits pten−/− fibroblast proliferation and migration.

Figure 7

(A) FN shRNA (Lane 1) stable transfection attenuates FN production in pten−/− cells, whereas control shRNA (Lane 2) has no effect. GAPDH is a loading control. MW denotes molecular weights of bands. (B) Control and FN shRNA-transfected pten−/− cells were assessed for proliferation using a commercially-available kit as described in Materials and Methods. FN knockdown resulted in an approximately 43% reduction in fibroblast proliferation compared to control shRNA-treated cells. Results are representative of 2 separate experiments. *p<0.005. (C) pten−/− fibroblast migration is attenuated following FN knockdown. Cells plated to confluence were scratched with a sterile 200 μl pipette tip and the wound area was measured every 2 hours. FN shRNA-treated fibroblasts (open circles) migrated significantly more slowly than control shRNA-transfected cells (closed squares). Results are representative of 2 separate experiments performed in triplicate wells. *p<0.05. **p<0.0005.