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. Author manuscript; available in PMC: 2011 Aug 20.
Published in final edited form as: Circ Res. 2010 Jun 24;107(4):532–539. doi: 10.1161/CIRCRESAHA.110.217075

Figure 1. Acute M119 treatment enhances cardiomyocyte β-AR signaling.

Figure 1

A) Isolated adult cardiomyocytes treated with M119 (10 μM) show enhanced cAMP generation at baseline and in response to Iso stimulation. B, C) M119 (10 μM) interferes with β-AR induced GRK2 membrane recruitment. Representative western blot analysis of GRK2 protein level in membrane fractions of cardiomyocytes treated with vehicle alone, Iso, M119, or M119 and Iso. Positive control lane of proteins from HEK293 cells transfected with GRK2 (B). Densitometric analysis of GRK2 membrane recruitment from four independent experiments demonstrates that M119 leads to ~50% decrease in Iso-induced GRK2 membrane recruitment (C). *P<.05, **P<.01, ***P<.001