Skip to main content
. Author manuscript; available in PMC: 2011 Aug 20.
Published in final edited form as: Cell. 2010 Aug 12;142(4):625–636. doi: 10.1016/j.cell.2010.07.019

Figure 3. Apoptosis inhibition by Bcl2 suppressed a subset of Bcl-x regulators.

Figure 3

(A) Screen hits (lightning bolts) may affect Bcl-x AS by many potential mechanisms. Regulation may be direct, such as (1) direct suppression of Bcl-xS formation or (2) facilitation of Bcl-xL. Indirect or downstream regulators may (3) promote cell survival pathways or (4) suppress pro-apoptotic pathways. We predicted that Bcl2 overexpression would suppress the splicing regulatory effect of siRNAs targeting indirect mechanisms, but not direct regulators.

(B) Bcl2 overexpression did not suppress the effect of SF3B1 depletion, consistent with direct regulation of Bcl-x splicing.

(C) Bcl2 suppressed the effect of siRNAs against mRNA export factors NXF1 and NUPL2, but not AURKA, on Bcl-x splicing (compare Venus images between ‘wild-type’ and ‘Bcl2 overexpression’). By contrast, Bcl2 suppressed cell death in all cases (compare DAPI images).

(D) 33% were suppressed by Bcl2 overexpression, while 67% showed partial to no suppression. GO functional enrichments within these subsets are shown with p-values (see Table S3).