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. Author manuscript; available in PMC: 2011 Aug 20.
Published in final edited form as: Cell. 2010 Aug 20;142(4):590–600. doi: 10.1016/j.cell.2010.07.018

Figure 7. Cells lacking ATG12-ATG3 exhibit decreased cell death mediated by mitochondrial pathways.

Figure 7

Cells were treated for 24h with the following agents: (A) 100 µM CCCP; (B) 100nM staurosporine; and (C) 20ng/mL TNF-α + 2.5µg/mL cycloheximide. Percent cell death (mean+/−SEM) was assayed by propidium iodide uptake using flow cytometry. (D) Lysates prepared from WTATG3 and KR cells were immunoblotted for the indicated markers. (E) Cells were treated for 24h with 500 nM obatoclax. Cell death (mean +/−SEM) was quantified using trypan blue exclusion. Statistical significance calculated using t test. (F) Summary model of results.