. Author manuscript; available in PMC: 2011 Aug 6.

Published in final edited form as: J Mol Biol. 2010 Jun 9;401(1):33–44. doi: 10.1016/j.jmb.2010.06.001

Table 2.

Pre-steady state kinetics of extension from a mismatched primer terminus

Mismatch	RT	k_pol (s⁻¹)^a	K_d (µM)^a	k_pol/K_d (µM⁻¹ s⁻¹)^a	Mismatched Extension Fidelity^b,^c	Fold Change to Wild Type
A:G	WT	1.06 ± 0.325	42.1 ± 5.37	0.0245 ± 0.0049	129
	K65R	1.45 ± 0.876	543.3 ± 45.11	0.0028 ± 0.0018	436	3
	K65A	0.239 ± 0.076	2175 ± 910.4	0.00011 ± 0.00006	3987	31

G:T	WT	34.3 ± 10.6	369.9 ± 14.2	0.0923 ± 0.0250	35
	K65R	14.2 ± 0.025	251.9 ± 36.1	0.0571 ± 0.0083	22	0.6
	K65A	7.02 ± 0.264	934.0 ± 254.6	0.0078 ± 0.0024	60	1.7

Values represent averages and standard deviations from at least two independent experiments.

Calculated as [(k_pol/K_d)_matched + (k_pol/K_d)_mismatched]/(k_pol/K_d)_mismatched

Compared to (k_pol/K_d)_matched from Table 1