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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Lab Invest. 2010 May 17;90(9):1365–1372. doi: 10.1038/labinvest.2010.89

Figure 5.

Figure 5

Effect of MMP2 inhibition on apoptosis of bovine retinal endothelial cells. (a) Apoptosis was measured by performing cell death ELISA by measuring cytoplasmic histone-associated-DNA- fragments using an assay kit from Roche Diagnostics. (b) PARP activation was measured by western blot technique, and the ratio of active PARP and pro-PARP was calculated. Each experiment was repeated using three separate cell preparation and the values obtained from the cells incubated in 5 mM glucose are considered as 100% (control). *P < 0.05 compared to the values obtained from the cells incubated in 5mM glucose. 5mM and 20mM=cells incubated in 5mM glucose, or 20mM glucose respectively, si and SC= cells transfected with MMP2-siRNA or scrambled RNA respectively, followed by incubation in 20mM glucose for 4 days.