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. 2010 Jul 6;1(3):e00102-10. doi: 10.1128/mBio.00102-10

FIG 2 .

FIG 2

Beta-lactam protection in a polymicrobial biofilm. Stationary biofilms of H. influenzae Rd and/or M. catarrhalis were established on chamber slides for 24 h and treated with 100 µg/ml ampicillin or ampicillin with 25 µg/ml clavulanate for an additional 24 h. Biofilms were resuspended in sterile PBS, serially diluted, and plated on sBHI medium plus clarithromycin and BHI medium plates for enumeration of viable H. influenzae Rd and M. catarrhalis bacteria, respectively. Data are represented as means ± SEM. *, P < 0.05 compared to H. influenzae plus ampicillin; **, P < 0.05 compared to polymicrobial H. influenzae plus ampicillin; ***, P < 0.05 compared to M. catarrhalis plus ampicillin.