Figure 1.

Electrophoretic patterns obtained in the purification of mTGR. Protein samples from the various steps of the purification protocol were incubated in the presence of 1% (p/v) SDS and 3% (v/v) β-mercaptoethanol and subjected to denaturing electrophoresis in 12% polyacrylamide gels containing 1% SDS. After run, gels were stained and destained by conventional procedures. Lanes are as follows: (1) crude extract; (2) DEAE-sephacel anion exchange chromatography; (3) Hydroxyapatite chromatography; (4) Cibacron-Blue affinity chromatography; and (5) molecular weight markers.