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. 2010 Jun 22;2010:719856. doi: 10.1155/2010/719856

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Copyright © 2010 Alberto Guevara-Flores et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Disulfide reductase activities and specificity of mTGR. Enzyme activities were carried out at 25°C as described under Materials and Methods. (a) Saturation kinetics with different disulfides. Initial velocity data were fitted to a hyperbolic saturation kinetic function with the aid of Sigma plot. The scale of the lower abscissa represent concentration of either GSSG or DTNB, while the upper-abscissa scale corresponds to Trx concentration. (▲) GSSG reductase activity; () Trx reductase activity with T. crassiceps Trx; (Δ) Trx reductase activity with DTNB. (b) Specificity of mTGR toward exogenous Trx. The reductase activity of mTGR was assayed in the presence of a variety of prokaryotic and eukaryotic Trx. A final concentration of 50 μM of the corresponding Trx was used. Abbreviations are as follows. Tc, Taenia crassiceps Trx; Pf: Plasmodium falciparum Trx; Hs, Homo sapiens Trx; Ec: Escherichia coli Trx; S, Spirulina sp Trx.

Inline graphic — Disulfide reductase activities and specificity of mTGR. Enzyme activities were carried out at 25°C as described under Materials and Methods. (a) Saturation kinetics with different disulfides. Initial velocity data were fitted to a hyperbolic saturation kinetic function with the aid of Sigma plot. The scale of the lower abscissa represent concentration of either GSSG or DTNB, while the upper-abscissa scale corresponds to Trx concentration. (▲) GSSG reductase activity; () Trx reductase activity with T. crassiceps Trx; (Δ) Trx reductase activity with DTNB. (b) Specificity of mTGR toward exogenous Trx. The reductase activity of mTGR was assayed in the presence of a variety of prokaryotic and eukaryotic Trx. A final concentration of 50 μM of the corresponding Trx was used. Abbreviations are as follows. Tc, Taenia crassiceps Trx; Pf: Plasmodium falciparum Trx; Hs, Homo sapiens Trx; Ec: Escherichia coli Trx; S, Spirulina sp Trx.