Table 1.
Summary of the mTGR purification procedure.
| Fraction | VolTotal (ml) | Protein (mg) | Specific Activity (mU*/mg) | Activity ratio | Activity (mU) | Purication (fold) | Yield (%) | ||
|---|---|---|---|---|---|---|---|---|---|
| GSSG | DTNB | HED | DTNB/GSSG | DTNB | |||||
|
| |||||||||
| Crude extract | 13.0 | 33.5 | 11.7 | 35,0 | 128.5 | 3.0 | 1172.5 | 1.0 | 100 |
| DEAE-Sephacel | 3.3 | 9.37 | 34.0 | 75.2 | 138.0 | 22 | 704.6 | 2.1 | 60 |
| HA-Ultrogel | 3.2 | 2.23 | 38.7 | 184.0 | 532.1 | 4.7 | 410.3 | 5.3 | 35 |
| Cibacron-Blue | 1.72 | 0.186 | 229.0 | 1091.0 | 2242.8 | 4.7 | 202.9 | 31.2 | 17 |
An enzyme mU is defined as the amount of protein needed to oxidize one nmol of NADPH per minute at 25°C.
Date obtained at 25°C and pH 7.8.