Fig. 4.

Macroscopic characteristics of ‘control’, ‘IRP1_mut’ and ‘IRP1_wt’ tumors. Nude mice were injected subcutaneously in each flank with parent H1299, HIRP1_mut or HIRP1_wt cells and tumor xenografts (arrows) were grown for 10 weeks. (A) Anesthetized animals from one representative experiment bearing ‘control’, ‘IRP1_mut’ and ‘IRP1_wt’ tumors prior to sacrifice. (B) and (C) Comparison of mass (B) and size (C) of isolated tumor xenografts from three independent experiments (mean ± SEM). A total of 17 tumor xenografts were formed from parent H1299 cells (n = 9 mice), 10 from HIRP1_wt cells (n = 6 mice) and 15 from HIRP1_mut cells (n = 9 mice). **P < 0.01 versus control (Student’s t-test).