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C-di-GMP and Mg2+ induced size and shape changes of the riboswitch, monitored by SAXS. Red indicates 2.5 mM Mg2+; black 10 mM Mg2+. Dots denote c-di-GMP-free conditions; solid lines, c-di-GMP-bound conditions ("a.u.", arbitrary units). (a) Kratky plot suggests local disorder of the ligand-free riboswitch even under high MgCl2 concentration (black dots). (b) Electron-pair probability analysis reveals compaction of the riboswitch upon binding the second messenger.
