Table 2.
The effect of TNFα on ABCA1, ABCG1, SR-BI, adiponectin, lipoprotein lipase (LPL) and PPARγ mRNA expression in 3T3L1 cells during adipocyte differentiation. The ΔCt value between gene of interest and house-keeping β-actin gene is presented.
| ΔCt for gene of interest |
Pre-adipocyte | Partially (~50%) differentiated |
Mature adipocyte | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Control | TNFα | P | Control | TNFα | P | Control | TNFα | P | |
| ABCA1 | 4.7±0.1 | 4.9±0.1 | ns | 2.2±0.3 | 2.6±0.1 | ns | 1.5±0.2 | 3.7±0.7 | * |
| ABCG1 | 9.0±0.3 | 10.2±0.2 | * | 5.5±0.1 | 6.4±0.2 | * | 4.0±0.1 | 5.9±0.6 | * |
| SR-BI | 5.2±0.2 | 6.4±0.1 | ** | 0.9±0.1 | 2.1±0.1 | *** | 0.2±0.2 | 1.7±0.4 | * |
| Adiponectin | 13.1±1.1 | 14.8±1.4 | ns | −3.1±0.4 | −1.9±0.1 | * | −2.7±0.3 | −1.9±0.2 | ns |
| LPL | 0.5±0.4 | 1.9±0.03 | * | −4.8±0.2 | −3.3±0.1 | ** | −4.6±0.2 | −3.8±0.2 | * |
| PPARγ | 6.1±0.1 | 7.9±0.2 | ** | 1.1±0.1 | 2.1±0.1 | ** | 0.9±0.1 | 1.7±0.1 | ** |
The lower the ΔCt value, the greater the mRNA expression (*p<0.05, **p<0.01 and ***p<0.001 vs. control, n=3).