Skip to main content
. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Glia. 2010 Sep;58(12):1395–1406. doi: 10.1002/glia.21015

Figure 5. Effect of 1-butanol and tert-butanol on carbachol-treated astrocytes-induced hippocampal neuron neuritogenesis.

Figure 5

Hippocampal neurons were incubated in the presence of astrocytes previously treated with 1 mM carbachol, 1-butanol (5 mM), and/or tert-butanol (5 mM) for 24 h. Morphometric analysis was carried out in neurons fixed and immunostained with a neuron-specific βIII-tubulin antibody. Pictures were taken with a digital camera attached to a fluorescence microscope. Quantifications of the length of the longest neurite (A) and the length of minor neurites (B) were carried out using the software MetaMorph. C: number of processes per cell. The results (mean +/− S.E.) derive from the measurements of 50–70 cells per treatment. *: p<0.05 vs control; #: p<0.05 vs carbachol by the Dunnett post-hoc test.