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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Glia. 2010 Sep;58(12):1395–1406. doi: 10.1002/glia.21015

Figure 6. Effect of 1-butanol and tert-butanol on carbachol-induced increase in extracellular fibronectin, laminin and PAI-1.

Figure 6

Astrocytes were stimulated for 24 h with 1 mM carbachol alone or in the presence of 1-butanol (5 mM) or tert-butanol (5 mM). After 24 h, astrocytes were fixed and immunolabeled with a fibronectin (A) or a laminin (B) antibodies followed by a fluorescent secondary antibody and analyzed by confocal microscopy. A: Average fibronectin immunofluorescence per cell. B: Average laminin immunofluorescence per cell. C: Media from astrocytes stimulated with 1 mM carbachol alone or in the presence of 1-butanol (5 mM) or tert-butanol (5 mM) were collected and analyzed for PAI-1 levels by ELISA. *: p<0.05, vs. control; #: p<0.05 vs. carbachol by the Dunnett post hoc test (n=6).