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. Author manuscript; available in PMC: 2010 Aug 23.
Published in final edited form as: J Neurovirol. 2007 Aug;13(4):315–327. doi: 10.1080/13550280701361508

Figure 3.

Figure 3

Cytopathic effects of HIV-1 primary isolates in MT-2 and U87.CD4 cell lines. Cytopathic effects for lymphocyte tropism was determined in the MT-2 cell line with the uninfected negative control (A) and the T-tropic isolate as positive control, HIV-1LAI (B). Isolates were characterized as not syncytia-inducing (NSI) (isolate 9) (C) or syncytia-inducing (SI) (isolate 12) (D) variants. Syncytia formation was also used as a parameter to determine coreceptor usage in the U87.CD4 glioma cells with laboratory-adapted isolates HIV-1LAI (H) and HIV-1BAL (I) used as positive controls for the U87.CD4.CXCR4 and U87.CD4.CCR5 lines, respectively. The negative control of the assay is shown in (E). Cells were screened every 3 days for cytopathic effects on U87.CD4.CCR5 (isolate 11) (F) and U87.CD4.CXCR4 (isolate 3) (G) lines. Data are representative from three different experiments performed in duplicate.