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. Author manuscript; available in PMC: 2010 Aug 23.
Published in final edited form as: J Immunol. 2009 Oct 21;183(10):6313–6319. doi: 10.4049/jimmunol.0803422

Figure 1. UCP2 is expressed in mouse and human mast cells.

Figure 1

(A). RT-PCR for UCP2 and β-actin. Lane 1, cDNA from BMMCs; lane 2, pooled cDNA from mouse brown fat, liver, brain and muscle for positive control; lane 3, BMMC mRNA not reverse transcribed for negative control. Images are representative of three separate experiments with similar results. (B) Immunoblot of lysate from wild-type BMMCs, Ucp2−/− BMMCs and LAD2 cells using antibody against UCP2. Immunoblot is representative of three experiments with similar results. (C) Representative May-Grünwald/Giemsa staining of wild-type and Ucp2−/− BMMCs. Original magnification, 100x. (D) Flow cytometry of the surface expression of c-Kit and FcεRI in wild-type and Ucp2−/− BMMCs. Dotted lines are BMMCs incubated with isotype control antibody. Data is representative of three experiments with similar results.

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