Figure 2. UCP2-deficient mast cells have enhanced degranulation.
(A) Wild-type and Ucp2−/− BMMCs were sensitized overnight with anti-DNP IgE (100 ng/ml) and challenged for 20 min with DNP-HSA (10 ng/ml). (B) Wild-type and Ucp2−/− FSMCs were stimulated for 10 min with SP (100 μM), C48/80 (30 μg/ml), or ionomycin (1 μM), after which histamine was measured in the supernatant and pellet. *P <0.05 (n=6)