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. Author manuscript; available in PMC: 2010 Aug 23.
Published in final edited form as: J Immunol. 2009 Oct 21;183(10):6313–6319. doi: 10.4049/jimmunol.0803422

Figure 3. Overexpression of UCP2 in LAD2 cells inhibits histamine release.

Figure 3

LAD2 cells were transduced with either a control lentivirus or UCP2 overexpression lentivirus and used for experiments 5 days after transduction. (A) Immunoblot of lysate from transduced LAD2 cells showing UCP2 overexpression. (B) Transduced LAD2 cells were sensitized overnight with human IgE (2 μg/ml) and challenged for 20 min with anti-IgE (10 μg/ml), or (C) stimulated for 10 min with SP (1 μM). *P <0.05 (t-test) vs. cells transduced with control virus. All data are presented as percent histamine release and represent the mean ± SD (n=3).

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