Table 1.

Observed rate (k_obs) of WT dCK, R104X-D133A double mutants, and human TK2 (sec⁻¹)

	Nucleoside	WT dCK	R104M-D133A	R104L-D133A	R104I-D133A	R104Q-D133A	TK2
A	D-dC	0.040a±0.001b	1.84±0.04c	0.21±0.09c	0.06±0.01c	0.08±0.01c	0.44±0.02
	D-dT	0.008±0.001c	1.15±0.01c	2.86±0.09c	0.40±0.01c	0.10±0.06c	0.70±0.04
	L-dT	0.017±0.002c	1.95±0.09c	1.07±0.09c	0.19±0.02c	0.15±0.02c	1.50±0.02
	D-dU	0.021±0.002	0.370±0.005	1.84±0.08	0.55±0.07	0.100±0.005	0.57±0.01
	L-dU	0.027±0.001	0.59±0.02	2.38±0.13	0.51±0.06	0.140±0.001	0.90±0.02
B	5-Me-dC	0.080±0.004	0.280±0.013	0.180±0.014	0.027±0.001	0.110±0.022	0.260±0.014
	5-Bromo-dC	0.050±0.001	0.250±0.001	0.076±0.017	0.012±0.005	0.060±0.004	0.250±0.010
	5-Iodo-dC	0.031±0.007	0.160±0.013	0.086±0.002	0.023±0.001	0.070±0.003	0.145±0.209
	5-Propynyl-dC	0.014±0.002	0.380±0.001	0.115±0.006	0.023±0.002	0.027±0.002	0.093±0.009
C	BVdU	0.010±0.001	0.57±0.06	1.16±0.08	0.190±0.040	0.100±0.020	0.180±0.013

^ak_obs are for a nucleoside concentration of 200 μM, plus 1 mM ATP

^bStandard deviation

^cData from Hazra et al, Biochemistry, 2009.