Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The NS5BΔ21 protein interacts with the full-length human ATG5 in yeast two-hybrid assay. NS5B and ATG5 fused to the Gal4 DNA binding and activation domains, respectively, were double-transformed into AH109 cells. Four independent colonies of recombinant AH109 cells were allowed to grow for a few days on −Leu, −Trp SD medium (left panel), after which they were replica-plated onto −Leu, −Trp, −His, −Ade + X-αgal plates (right panel).
