Fig. 3.

Mapping of the NS5BΔ21 and ATG5 binding domains. Results are indicated in the column on the right and were obtained by yeast two-hybrid assay (first two columns) or by co-IP (third column). Deletion mutants of NS5B were assessed for interaction with full-length ATG5. The binding area covered the N-terminal amino acids 450–570 as indicated by yeast two-hybrid assay and co-IP. Note that none of the deletion mutants of NS5BΔ21 self-activated in the yeast two-hybrid screen, as shown in the second column. nd indicates not done.