Fig. 4.

A. Huh7 and C5B cells were grown for 48 h and costained with antibodies specific to ATG5 and NS4B protein. All samples were examined via confocal microscopy. Magnified areas (a) are indicated by rectangles. Notice the colocalization of NS4B with ATG5 in C5B cells. B. Subcellular fractionation of clone A cells (subgenomic replicon) transfected with GFP-ATG5. Proteins were resolved on SDS–PAGE and visualized by Western blot using a polyclonal rabbit anti-NS5B (left) or a monoclonal anti-GFP (right). N, nucleus; C, cytoplasm; M/M, microsome and mitochondria.