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GADD34 expression is elevated in aged animals and further increased by sleep deprivation. Top, Representative Western blot showing samples (20 μg of protein) from young and aged animals after 6 h of sleep deprivation (SD) and their diurnal controls (Con). Bottom, Bar graph illustrating increased GADD34 expression in aged animals quantified from Western blots (n = 5; *p = 0.005).
