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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Exp Hematol. 2009 Sep 20;37(12):1387–1392. doi: 10.1016/j.exphem.2009.09.003

Figure 1.

Figure 1

A. The hCD68-rtTA-M2 construct. The 4.3 kbp construct includes the 2.9 Kbp genomic DNA immediately upstream of the start site, followed by the 83 bp fist intron (IVIS), the rtTA-M2, and the bovine growth hormone poly A tail.

B. Demonstration of GFP in hematopoietic tissue of double transgenic animals induced with doxycycline (induced) or without doxycycline (control). BM – Bone Marrow, PB-Peripheral Blood, SPLN- Spleen. About 10% of all bone marrow cells were positive for GFP, while only 0.2 % of cells were positive in peripheral blood and 1% in spleen. Negligible numbers of cells were GFP-positive in the control animals. When GFP-positive cells were superimposed (green) onto FSC-SSC contour plots, they distributed to a pattern expected of monocytes-macrophages.