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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Exp Hematol. 2009 Sep 20;37(12):1387–1392. doi: 10.1016/j.exphem.2009.09.003

Figure 2. Identifying the lineage of the GFP-expressing cells in bone marrow by flow-cytometry.

Figure 2

Gr-1 and Cd11b are markers of the myeloid lineage; the GFP-expressing cells were mostly contained in the Gr-1 and Cd11b fraction of cells. While most of the GFP-expressing cells also expressed F4/80, about 25% of GFP-expressing cells were F4/80-negative, suggesting that some mature macrophages are negative for F4/80 in hematopoietic tissue. The GFP-expressing cells were also analyzed for markers of other lineages including Thy1.2 (T-lymphocyte), B220 (B-lymphocyte) and Ter-119 (erythrocyte).