Table 4.
Effect of Centella asiatica (CA; 150 and 300 mg/kg, PO) on colchicines-induced oxidative stress parameters in rat brain.
| Treatment (mg/kg) | MDA levels nmol MDA/mg protein (% of sham) | Nitrite levels μmol/mg protein (% of sham) | Reduced glutathione nmol/mg protein (% of sham) | Catalase μmol of hydrogen peroxide decomposed/min/mg protein ( % of sham) | Superoxide dismutase Units/mg protein (% of sham) | Glutathione- S- transferase nmol of CDNB conjugated/min/mg protein (% of sham) |
|---|---|---|---|---|---|---|
| Sham | 100 ± 10 | 100 ± 12 | 100 ± 10 | 100 ± 12 | 100 ± 14 | 100 ± 12 |
| ACSF | 114.28 ± 11 | 109.33 ± 11 | 103.33 ± 11 | 97.05 ± 10 | 97.52 ± 15 | 98.66 ± 16 |
| COL | 339.88 ± 32a | 298.39 ± 15a | 23.38 ± 10a | 17.98 ± 8a | 13.47 ± 3a | 18.67 ± 7a |
| CA (150) | 106.7 ± 15 | 101.29 ± 16 | 90.35 ± 17 | 93.9 ± 13.7 | 99.17 ± 17 | 97.64 ± 14 |
| CA (300) | 123.4 ± 28 | 99.44 ± 26 | 93.77 ± 15 | 98.8 ± 10.8 | 98.16 ± 16 | 98.65 ± 13 |
| CA (150) + COL | 229.81 ± 27b | 210.91 ± 8b | 50.45 ± 8b | 36.96 ± 6b | 39.05 ± 10^b | 30.65 ± 8b |
| CA (300) + COL | 157.26 ± 16b,c | 148.98 ± 7.9b,c | 81.61 ± 7.6b,c | 69.71 ± 5b,c | 65.52 ± 5b,c | 65.05 ± 6b,c |
Values are mean ± SEM; a P < .05 as compared to artificial cerebrospinal fluid- (ACSF-) injected group; b P < .05 as compared to colchicine-injected group; c P < .05 as compared to CA (150) + COL group; (repeated measures two-way ANOVA followed by Tukey's test for multiple comparisons).
Note: ACSF: artificial cerebrospinal fluid; COL: colchicine; CA: Centella asiatica.