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. 2010 May 20;2010:798424. doi: 10.4061/2010/798424

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Copyright © 2010 Erik Hjorth et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a)-(b) Effects of incubation with interleukin-1β (IL-1β) and interferon-γ (IFNγ), added 24 hours before incubation with 1 μg/mL Aβ _1–42 (black boxes) or vehicle (white boxes), on secreted levels of IL-6 (a) and BDNF (b) from CHME3 microglial cells. The cells were incubated with 1 μg/mL HiLyte488-conjugated Aβ _1–42 following prestimulation for 24 hours with 50 ng/mL IL-1β and 50 ng/mL IFNγ. The cells were harvested at 24, 48, 72, and 96 hours after addition of Aβ _1–42. The data are expressed as % uptake of control vehicle set at 100% and shown as median ± percentiles (25%–75% and 10%–90%), n = 4 for 24 hours and 48 hours and n = 6 for 72 and 96 hours. A statistically significant effect of treatment was found by Kruskal-Wallis ANOVA at 24 hours (P = .0087), at 72 hours (P = .004), and at 96 hours (P = .004). Statistical difference from control is indicated by **(P < .01); difference from Aβ _1–42 # is indicated by (P < .05) and ^##(P < .01). † indicates statistical difference (P < .05) between IL-1β and IL-1β + IFNγ.

(a)-(b) Effects of incubation with interleukin-1β (IL-1β) and interferon-γ (IFNγ), added 24 hours before incubation with 1 μg/mL Aβ _1–42 (black boxes) or vehicle (white boxes), on secreted levels of IL-6 (a) and BDNF (b) from CHME3 microglial cells. The cells were incubated with 1 μg/mL HiLyte488-conjugated Aβ _1–42 following prestimulation for 24 hours with 50 ng/mL IL-1β and 50 ng/mL IFNγ. The cells were harvested at 24, 48, 72, and 96 hours after addition of Aβ _1–42. The data are expressed as % uptake of control vehicle set at 100% and shown as median ± percentiles (25%–75% and 10%–90%), n = 4 for 24 hours and 48 hours and n = 6 for 72 and 96 hours. A statistically significant effect of treatment was found by Kruskal-Wallis ANOVA at 24 hours (P = .0087), at 72 hours (P = .004), and at 96 hours (P = .004). Statistical difference from control is indicated by **(P < .01); difference from Aβ _1–42 # is indicated by (P < .05) and ^##(P < .01). † indicates statistical difference (P < .05) between IL-1β and IL-1β + IFNγ.