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. 2010 May 20;2010:798424. doi: 10.4061/2010/798424

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Copyright © 2010 Erik Hjorth et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of interleukin-1β (IL-1β) and interferon-γ (IFNγ) on cell viability in human CHME3 microglial cells treated with Aβ _1–42 (grey boxes) or vehicle (white boxes). The cells were preincubated with 50 ng/mL IL-1β and 50 ng/mL IFNγ for 24 hours prior to addition of Aβ _1–42 (1 μg/mL). At 24, 48, 72, and 96 hours after addition of Aβ _1–42 the viability of the cultures was assessed with the MTT assay. The data are expressed as % of control (vehicle) set at 100% and shown as median ± percentiles (25%–75% and 10%–90%), n = 4 (24, 48 h), n = 6 (72, 96 h). Statistical difference from vehicle is indicated by *(P < .05) and ***(P < .005), and statistical difference from Aβ _1–42 is indicated by ^#(P < .05) and ^##(P < .01). (P < .01) indicates statistical difference between IFNγ and IL-1β + IFNγ, and ^†(P < .05) indicate differences between IL-1β and IL-1β + IFNγ.

Inline graphic — Effects of interleukin-1β (IL-1β) and interferon-γ (IFNγ) on cell viability in human CHME3 microglial cells treated with Aβ _1–42 (grey boxes) or vehicle (white boxes). The cells were preincubated with 50 ng/mL IL-1β and 50 ng/mL IFNγ for 24 hours prior to addition of Aβ _1–42 (1 μg/mL). At 24, 48, 72, and 96 hours after addition of Aβ _1–42 the viability of the cultures was assessed with the MTT assay. The data are expressed as % of control (vehicle) set at 100% and shown as median ± percentiles (25%–75% and 10%–90%), n = 4 (24, 48 h), n = 6 (72, 96 h). Statistical difference from vehicle is indicated by *(P < .05) and ***(P < .005), and statistical difference from Aβ _1–42 is indicated by ^#(P < .05) and ^##(P < .01). (P < .01) indicates statistical difference between IFNγ and IL-1β + IFNγ, and ^†(P < .05) indicate differences between IL-1β and IL-1β + IFNγ.