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. Author manuscript; available in PMC: 2010 Aug 23.
Published in final edited form as: J Stem Cells. 2010;4(4):203–216.

Figure 5.

Figure 5

The effect of nicotine on chemokine-mediated migration of hMSCs. A: Chemotaxis of hMSCs towards plain culture media or media containing 0.2% BSA or the same media supplemented with 20 ng/ml bFGF or 100 nM C3a was evaluated using a Transwell assay. Where indicated, 10−6M nicotine and/or 100 nM α-bungarotoxin was added into the Transwell cultures. Each sample was assayed in triplicates. Statistical differences are indicated by * for p values of <0.05 (Student’s t test). Results of one out of two similar experiments are shown. B: SCID mice were irradiated with 6Gy followed by intravenous infusion of 3×105 hMSCs expressing GFP (GFP-hMSCs). After 24 hours, bone marrow, spleens and lungs were collected and the number of GFP-hMSCs was measured by FACS. The number of GFP-hMSCs in control animals is presented as 100%. The data shown are the average (±SD) of three independent measurements.