. Author manuscript; available in PMC: 2011 Mar 27.

Published in final edited form as: Tetrahedron. 2010 Mar 27;66(13):2277–2283. doi: 10.1016/j.tet.2010.01.067

Table 2.

NCL at threonine through ligation and subsequent free-radical desulfurization



Entry	Peptide A	Ligation Product/Yield/Time	Desulfurization Product/Yield/Time
1			FmocRLGDSTAGYTGAPRHSWG—OMe 39 / 98% / 2 h
2			FmocRLGDSTAGWTGAPRHSWG—OMe 41 / 85% / 2 h
3			FmocRTGDSAGITGAPRHSWG—OMe 43 / 96% / 2 h
4

Key: (a) buffer at pH 6.5 (6.0 M Gn·HCl, 188.8 mM Na₂HPO₄), TCEP, RT; (b) TCEP, VA-044, tBuSH, 37 °C.