Table 2.
Experimental strategies for verifying ghrelin action.
| Genetic |
| (1) transgenic silencing of ghrelin gene |
| (2) transgenic silencing of ghrelin receptor |
| (3) double knockout |
| (4) antisense transgene to neuronal ghrelin receptor |
| (5) overexpression of ghrelin |
| Immunologic |
| (1) immunoneutralization |
| (2) catalytic antibodies |
| Antagonists |
| (1) peptides |
| (2) nonpeptides |
| (3) RNA Spiegelmer |
| Infusion of ghrelin or desacyl-ghrelin |
| (1) agonism and antagonism |
| Anatomic definition of ghrelin-neural network |
| (1) transgenic green fluorescent protein-linked ghrelin |