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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Cell Biochem. 2010 Mar 1;109(4):828–837. doi: 10.1002/jcb.22463

Figure 5. Runx2 interacts with AR and increases androgen activity and PSA production in LNCaP cells.

Figure 5

(A) Treatment of LNCaP cells with DHT (10 nM) and/or TGFβ (10 ng/ml) for 24 h did not affect AR or Runx2 expression at the protein level. (B) DHT (20 nM) does not significantly increase Runx2 activity in LNCaP cells. Cells were transfected with 50 ng, 100 ng and 200 ng Runx2-HA plasmid DNA or empty factor (EV) as a negative control. Luciferase activity was normalized to Renilla values. A representative experiment is shown from 3 independent experiments performed in triplicate. (C) Runx2 enhances ARE-activity in LNCaP cells in a dose-dependent manner. A representative experiment is shown from 3 independent experiments performed in triplicate. (D) Pulldown with Runx2 antibody in immunoprecipitation assay using LNCaP cell lysate demonstrates that Runx2 and AR physically interact. (E) DHT incubation (10 nM) for 24 h stimulates PSA protein production in LNCaP cells as measured by ELISA. (F) Forced Runx2-HA expression (250 ng, 500 ng, 1000 ng) in LNCaP cells strongly increases PSA production after 24 h of DHT (20 nM) treatment. A representative experiment is shown performed in triplicate.