Figure 6.
Leptin induces endometrial cancer cell invasion via AKT and JAK/STAT pathways. ECC1 were cultured in Matrigel invasion chambers in serum-free media containing 100 ng/ml leptin (‘L’). For combined treatment, cells were pretreated with 10 μM LY294002 for 45 min followed by leptin treatment (‘L+LY’) and 100 μM AG490 for 45 min followed by leptin treatment (‘L+AG’) for 24 h, and the number of cells that invaded through the Matrigel were counted. Each bar represents a Matrigel invasion assay performed in triplicate twice (means±s.e.m.). *P < 0.01, compared with untreated (‘U’) control cells; #P < 0.01, compared with leptin (‘L’) treatment.