Skip to main content
. Author manuscript; available in PMC: 2010 Aug 23.
Published in final edited form as: Cancer Res. 2007 Mar 15;67(6):2497–2507. doi: 10.1158/0008-5472.CAN-06-3075

Figure 6. Leptin up-regulates the migration of hepatocellular carcinoma cells.

Figure 6

A, both HepG2 and Huh7 cells were grown to confluence on fibronectin-coated surface, serum-starved for 16 h, and scratched with a pipette tip. The plates were photographed immediately following scratching. Culture media were replaced with control media (media containing 100 ng/mL leptin). For combined treatment, cells were pretreated with 100 µmol/L AG490 (Leptin + AG), 10 µmol/L PD98059 (Leptin + PD), or 10 µmol/L LY294002 (Leptin + LY) along with leptin treatment. The plates were photographed at the identical location of the initial image at 6 and 12 h. B, confluent HepG2 and Huh7 cells, seeded in ECIS plates and treated with leptin (100 ng/mL) alone or along with 100 µmol/L AG490 (AG + Leptin) or 10 µmol/L PD98059 (PD + Leptin) or 10 µmol/L LY294002 (LY + Leptin), were subjected to an elevated voltage pulse of 40-kHz frequency at 3.5-V amplitude for 30 s. The wound was then allowed to heal from cells surrounding the small active electrode that did not undergo the elevated voltage pulse. Resistance was measured before and after the elevated voltage pulse application as described in Materials and Methods. The measurements were stopped 24 h after wound healing. All the experiments were done thrice in triplicates.