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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Hepatology. 2010 May;51(5):1810–1819. doi: 10.1002/hep.23626

Figure 2. hHiPS cells can differentiate into all three primary germ layers in vitro and in vivo.

Figure 2

(a, b) Embryoid bodies derived from hHiPS 10, and 11 lines, respectively. (c–h) In vitro differentiation of hHiPS cells into all three primary germ cell layers. After generation of embryoid bodies hHiPS cells spontaneously differentiated into endoderm (c, d) (α-fetoprotein-positive, red), mesoderm (e, f) (smooth muscle actin-positive, red) and ectoderm (g, h) (TuJ1-positive neuronal cells, green). c, e, g: hHiPS10 and d, f, h: hHiPS11. Blue nuclear staining is DAPI. ×100, Scale bars, 100 µm. (i–n) Spontaneous differentiation into all three germ layers is evident in teratomas. (i, j) endoderm, (k, l) mesoderm, (m, n) ectoderm derived from hHiPS10 and hHiPS14. ×200, Scale bars, 50 µm.