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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Hepatology. 2010 May;51(5):1810–1819. doi: 10.1002/hep.23626

Figure 4. Differentiation of hHiPS cells into mature hepatocytes.

Figure 4

The differentiated cells possessed proteins indicative of functional hepatocytes, including ALB, green (a), ALB (green) + AAT (red) (b), and CYP3A4 (pink) (c) at day 20 after differentiation initiation. ×200, Scale bars, 100 µm. (d) Glycogen storage at day 20. Periodic acid-Schiff assay was performed on differentiating cells at 20 days post-initial Activin A treatment. Nuclei were counterstained with hematoxylin (blue). Glycogen storage is indicated by pink or dark red cytoplasm. ×100, Scale bar = 100 µm.