Table 2.
% of T cells IFN-γ+ | No stimulation | PHA | HPV16 E6 peptide 1–30 pool | HPV16 E7 peptide 1–18 pool |
---|---|---|---|---|
CD4+ | ||||
Macaque 746 | 0.002 | 0.53 | 0.009 | 0.013 |
Macaque 811 | 0.027 | 0.406 | 0.238 | 0.093 |
Macaque 831 | 0.001 | 0.128 | 0.099 | 0.134 |
CD8+ | ||||
Macaque 746 | 0.019 | 6.33 | 0.026 | 0.014 |
Macaque 811 | 0.052 | 5.060 | 0.278 | 0.091 |
Macaque 831 | 0.013 | 1.537 | 0.058 | 0.114 |
Pigtail macaques (numbers 746, 811 and 831) were immunized with 125 μg of TA-CIN with 1000 μg GPI-0100 at monthly intervals. PBMCs were harvested one month after the last vaccination and characterized for HPV16 E6- and E7-specific CD4+ (A) and CD8+ (B) T cells using intracellular IFN-γ staining and flow cytometric analysis after stimulation with overlapping peptide pools or as a positive control, PHA. The percentage of CD4 (A) or CD8 (B) positive PBMCs secreting IFN-γ are presented.