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. Author manuscript; available in PMC: 2010 Aug 23.
Published in final edited form as: Vaccine. 2008 Dec 16;27(7):1040–1049. doi: 10.1016/j.vaccine.2008.11.099

Table 2.

Vaccination of macaques with TA-CIN in GPI-0100 adjuvant induces HPV16 E6- and E7-specific CD4+ and CD8+ T cell responses.

% of T cells IFN-γ+ No stimulation PHA HPV16 E6 peptide 1–30 pool HPV16 E7 peptide 1–18 pool
CD4+
Macaque 746 0.002 0.53 0.009 0.013
Macaque 811 0.027 0.406 0.238 0.093
Macaque 831 0.001 0.128 0.099 0.134
CD8+
Macaque 746 0.019 6.33 0.026 0.014
Macaque 811 0.052 5.060 0.278 0.091
Macaque 831 0.013 1.537 0.058 0.114

Pigtail macaques (numbers 746, 811 and 831) were immunized with 125 μg of TA-CIN with 1000 μg GPI-0100 at monthly intervals. PBMCs were harvested one month after the last vaccination and characterized for HPV16 E6- and E7-specific CD4+ (A) and CD8+ (B) T cells using intracellular IFN-γ staining and flow cytometric analysis after stimulation with overlapping peptide pools or as a positive control, PHA. The percentage of CD4 (A) or CD8 (B) positive PBMCs secreting IFN-γ are presented.