Figure 3.

Expression patterns of KLFs 2, 4 and 6 in chicken embryos between stages 1–27. A–K) KLF2; D) higher magnification view of the atrioventricular canal region of the embryo in C); E) section through the atrioventricular canal region of the heart of the embryo in C). H) dorsal view of the embryo in G; J) dorsal view of the embryo in I; K) section through the leg of the embryo shown in J, arrowheads indicate faint labeling of the chondrogenic condensations. L–S) KLF4; O) ventral view of the facial structures of embryo in N); P,Q) dorsal and ventral views of the posterior region of embryo in N; R) section through leg region of embryo in Q) showing the location of label corresponding to the arrowhead and arrow in Q); S) transverse section through the left leg bud in Q), showing the dorsal (double arrowheads) and ventral labeling of mesoderm beneath the ectoderm. T-B’) KLF5; Y) transverse section of embryo in U) showing labeling of non neural ectoderm and lateral mesoderm; Z) transverse section through the posterior region of a stage 11 embryo, showing labeling of non neural ectoderm A’) Magnification of boxed region in Z). C’-G’) KLF6; G’) Transverse section through embryo in F’, showing myotome labeling (double arrowheads). See text for detailed descriptions of these expression patterns.