Figure 5.

(A) Representative Western blots of apoptosis-associated proteins. (B) Quantitative analysis of expression for apoptosis-associated proteins. The Western blotting analysis showed that the levels of caspase-3 and bax were significantly increased, while the level of Bcl-2 was significantly decreased in the control group, compared with the sham-operated dogs. Spironolactone treatment significantly reduced the up-regulation of caspase-3 and bax, and the down-regulation of Bcl-2 compared with control group. ^##P < 0.01, compared with the sham-operated group; *P < 0.05, **P < 0.01, compared with the control group.