Figure 7.

Effect of Ga on alkaline phosphatase (ALP) activity. Murine MC3T3-E1 osteoblastic cells (A, B) and murine primary calvaria-derived osteoblasts (C, D) were cultured for 12 days and 4 days respectively. Then, cells were treated with 100 µM Ga or 100 ng·mL⁻¹ BMP-2 (+) or their respective vehicles (−) for 48 h. Total protein content was determined with a Coomassie assay. Results are expressed as relative protein content as compared with untreated cells (A, C). ALP activity was spectrophotometrically measured. Results are expressed as relative ALP activity as compared with untreated cells (B, D). *P < 0.05 significantly different from corresponding untreated cells. Ga, gallium; BMP-2, bone morphogenetic protein-2.