Figure 2.

LASSBio-881 inhibition of CAP currents is surmountable in TRPV1-expressing oocytes. (A) Representative recording of LASSBio-881 inhibition of 1 µM CAP currents in TRPV1-expressing oocytes. (B) Concentration–response curve for LASSBio-881 against 1 µM CAP, normalized to 10 µM CAP applied at the end of recording. Estimated IC₅₀ was 14.05 µM (n= 3–7 oocytes per concentration). (C) Representative recording of a TRPV1 Δ777-820-expressing oocyte challenged with 50 nM CAP in the absence or presence of various concentrations of LASSBio-881. (D) Concentration–response curve for LASSBio-881 against 50 nM CAP, normalized to 10 µM CAP applied at the end of recording. Estimated IC₅₀ was 4.95 µM (n= 4–9 oocytes per concentration). (E) Concentration–response curves for TRPV1 currents induced by CAP in the absence and presence of 20 µM LASSBio-881. Estimated EC₅₀ for CAP were 347.8 nM and 2.06 µM, alone and with 20 µM LASSBio-881, respectively (n= 5 oocytes in each curve).