Figure 4.

Proteins and genes associated with metabolic processes dominate differential expression in evolved strains. Adaptively evolved strains have hundreds of altered gene and protein expression levels covering a broad range of COGs. However, the relatively large coverage of genes and proteins in COGs associated with metabolism (red brackets) clearly suggests the importance of changes in the metabolic network. The most significantly enriched metabolic COG is ‘carbohydrate metabolism and transport.’ In addition, the proteomic data suggest that the transcription and translation machinery may also have an important function in the physiological changes witnessed in the adaptively evolved strains.